Recombinant human Adenosylhomocysteinease/AHCY protein

AHCY, also known as adenosylhomocysteinase isoform 1, is an enzyme that catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy) . AdoHcy hydrolysis is a reversible reaction with an equilibrium favoring AdoHcy formation, but hydrolysis prevails under physiological conditions due to the rapid removal of adenosine and homocysteine. Thus, AHCYs activity in mammals is directly related to homocysteine level, an independent risk factor for vascular disease. It also functions as a regulator of biological transmethylation by controlling the concentration of AdoHcy, a potent competitive inhibitor of all S-adenosyl-L-methionine methyltransferases. Recombinant human AHCY protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Adenosylhomocysteinase isoform 1, AHCY, AdoHcyase, SAHH, S-adenosylhomocysteine hydrolase

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

48.8 kDa (441aa)

Additionnal Information

Adenosylhomocysteinease, AHCY, Adenosylhomocysteinase isoform 1, AHCY, adoHcyase, SAHH, ATGP3578-10 µg, ATGP3578-20 µg, ATGP3578-50 µg, ATGP3578-100 µg, ATGP3578-250 µg, ATGP3578-500 µg, ATGP3578-1 mg, ATGP3578-10, ATGP3578-20, ATGP3578-50, ATGP3578-100, ATGP3578-250, ATGP3578-500, ATGP3578-1

References & Citations

Vugrek O. et al., (2009) Hum Mutat. 30: E555-65.; ; Park SJ. et al., (2015) Am J Cancer Res. 5: 2127-2138.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_000678

Uniprot Accession Number

P23526

Species

Human

N Terminal Sequence

Ala

AA Sequence

MSDKLPY KVADIGLAAW GRKALDIAEN EMPGLMRMRE RYSASKPLKG ARIAGCLHMT VETAVLIETL VTLGAEVQWS SCNIFSTQDH AAAAIAKAGI PVYAWKGETD EEYLWCIEQT LYFKDGPLNM ILDDGGDLTN LIHTKYPQLL PGIRGISEET TTGVHNLYKM MANGILKVPA INVNDSVTKS KFDNLYGCRE SLIDGIKRAT DVMIAGKVAV VAGYGDVGKG CAQALRGFGA RVIITEIDPI NALQAAMEGY EVTTMDEACQ EGNIFVTTTG CIDIILGRHF EQMKDDAIVC NIGHFDVEID VKWLNENAVE KVNIKPQVDR YRLKNGRRII LLAEGRLVNL GCAMGHPSFV MSNSFTNQVM AQIELWTHPD KYPVGVHFLP KKLDEAVAEA HLGKLNVKLT KLTEKQAQYL GMSCDGPFKP DHYRY