Recombinant E.coli Beta-lactamase protein

AmpC, also known as Beta-lactamase, is the most widespread resistance mechanism to beta-lactam antibiotics, such as the penicillins and the cephalosporins. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule's antibacterial properties. Recombinant E. coli beta-lactamase protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Specific activity is >700 units/mg, in which One unit will hydrolyze 1.0umole of Nitrocefin per minute at pH 7.0 at 37C

Product Name Alternative

AmpC, ampA, Cephalosporinase

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is >700unit/mg, in which One unit will hydrolyze 1.0umole of Nitrocefin per minute at pH 7.0 at 37C

Molecular Weight

41.8 kDa (379aa) confirmed by MALDI-TOF

Additionnal Information

B-lactamase, ATGP3575-10 µg, ATGP3575-20 µg, ATGP3575-50 µg, ATGP3575-100 µg, ATGP3575-250 µg, ATGP3575-500 µg, ATGP3575-1 mg, ATGP3575-10, ATGP3575-20, ATGP3575-50, ATGP3575-100, ATGP3575-250, ATGP3575-500, ATGP3575-1

References & Citations

Zhao Z., et al. (2015) Cancer Lett. 358 (1) :17-26.; ; Martin I., et al. (2014) Cell. 157 (2) :472-85.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_418574

Uniprot Accession Number

P00811

Species

E.coli

AA Sequence

APQQINDIV HRTITPLIEQ QKIPGMAVAV IYQGKPYYFT WGYADIAKKQ PVTQQTLFEL GSVSKTFTGV LGGDAIARGE IKLSDPTTKY WPELTAKQWN GITLLHLATY TAGGLPLQVP DEVKSSSDLL RFYQNWQPAW APGTQRLYAN SSIGLFGALA VKPSGLSFEQ AMQTRVFQPL KLNHTWINVP PAEEKNYAWG YREGKAVHVS PGALDAEAYG VKSTIEDMAR WVQSNLKPLD INEKTLQQGI QLAQSRYWQT GDMYQGLGWE MLDWPVNPDS IINGSDNKIA LAARPVKAIT PPTPAVRASW VHKTGATGGF GSYVAFIPEK ELGIVMLANK NYPNPARVDA AWQILNALQ