Recombinant human CSTF1 protein

CSTF1, also known as cleavage stimulation factor subunit 1, is one of the multiple factors required for polyadenylation of mammalian pre-mRNAs in vitro. It is functioning with cleavage-polyadenylation specificity factor, two cleavage factors, and poly (A) + polymerase. It is involved in the 3' end processing of messenger RNA precursors. Recombinant human CSTF1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cleavage stimulation factor subunit 1, CSTF1, CstF-50, CstFp50

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

49.1 kDa (437aa)

Additionnal Information

CSTF1, Cleavage stimulation factor subunit 1, CSTF1, CstF-50, CstFp50, ATGP3573-10 µg, ATGP3573-20 µg, ATGP3573-50 µg, ATGP3573-100 µg, ATGP3573-250 µg, ATGP3573-500 µg, ATGP3573-1 mg, ATGP3573-10, ATGP3573-20, ATGP3573-50, ATGP3573-100, ATGP3573-250, ATGP3573-500, ATGP3573-1

References & Citations

Takagaki Y., et al. (1994) Nature. 372:471-474.; ; Ruegsegger U., et al. (1996) J Biol Chem. 271:6107-6113.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 1mM DTT, 20% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001028693

Uniprot Accession Number

Q05048

Species

Human

N Terminal Sequence

Met1

AA Sequence

MYRTKVGLKD RQQLYKLIIS QLLYDGYISI ANGLINEIKP QSVCAPSEQL LHLIKLGMEN DDTAVQYAIG RSDTVAPGTG IDLEFDADVQ TMSPEASEYE TCYVTSHKGP CRVATYSRDG QLIATGSADA SIKILDTERM LAKSAMPIEV MMNETAQQNM ENHPVIRTLY DHVDEVTCLA FHPTEQILAS GSRDYTLKLF DYSKPSAKRA FKYIQEAEML RSISFHPSGD FILVGTQHPT LRLYDINTFQ CFVSCNPQDQ HTDAICSVNY NSSANMYVTG SKDGCIKLWD GVSNRCITTF EKAHDGAEVC SAIFSKNSKY ILSSGKDSVA KLWEISTGRT LVRYTGAGLS GRQVHRTQAV FNHTEDYVLL PDERTISLCC WDSRTAERRN LLSLGHNNIV RCIVHSPTNP GFMTCSDDFR ARFWYRRSTT DHHHHHH