Recombinant mouse PGP9.5/UCHL1 protein
Uchl1 also known as Ubiquitin carboxyl-terminal hydrolase isozyme L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. UCHL1 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. Recombinant mouse Uchl1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Specific activity is > 70 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of ubiquitin-AMC per minute at pH 7.5, at 37C
Product Name Alternative
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Specific activity is > 70pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0pmole of ubiquitin-AMC per minute at pH 7.5, at 37C
Molecular Weight
27.2 kDa (246aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Wiese CB., et al. (2013) Genesis 51 (12), 852-861
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT
Scientific Category
Neuroscience
NCBI Accession Number
NP_035800
Uniprot Accession Number
Q9R0P9
Species
Mouse
AA Sequence
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