Recombinant human ECHS1 protein

Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, also known as ECHS1, is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Expressed in muscle, liver and fibroblasts, with low expression in kidney and spleen, ECHS1 exists as a homohexamer that functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. Recombinant human ECHS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 150 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 umole of crotonoyl-CoA to hydroxybutyryl-CoA per minute per minute at pH 7.5 at 25C.

Product Name Alternative

Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, SCEH

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

Enzyme Activity, SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 150unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 umole of crotonoyl-CoA to hydroxybutyryl-CoA per minute per minute at pH 7.5 at 25C.

Molecular Weight

30.6 kDa (284aa) confirmed by MALDI-TOF

Additionnal Information

ECHS1, Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, SCEH, ATGP3567-10 µg, ATGP3567-20 µg, ATGP3567-50 µg, ATGP3567-100 µg, ATGP3567-250 µg, ATGP3567-500 µg, ATGP3567-1 mg, ATGP3567-10, ATGP3567-20, ATGP3567-50, ATGP3567-100, ATGP3567-250, ATGP3567-500, ATGP3567-1

References & Citations

Kanazawa M., et al. (1993) Enzyme Protein. 47:9-13.; ; Stern J R., et al. (1956) J Biol Chem. 218:985-1002.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 100 mM NaCl

Scientific Category

Metabolism

NCBI Accession Number

AAH08906

Uniprot Accession Number

P30084

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MASGANFEYI IAEKRGKNNT VGLIQLNRPK ALNALCDGLI DELNQALKIF EEDPAVGAIV LTGGDKAFAA GADIKEMQNL SFQDCYSSKF LKHWDHLTQV KKPVIAAVNG YAFGGGCELA MMCDIIYAGE KAQFAQPEIL IGTIPGAGGT QRLTRAVGKS LAMEMVLTGD RISAQDAKQA GLVSKICPVE TLVEEAIQCA EKIASNSKIV VAMAKESVNA AFEMTLTEGS KLEKKLFYST FATDDRKEGM TAFVEKRKAN FKDQ