Recombinant human M-CSF protein

CSF1, also known as macrophage colony-stimulating factor 1, is a hematopoietic growth factor that is the primary regulator of the survival, proliferation, differentiation and function of mononuclear phagocytes. It is a four alpha-helical bundle cytokine and its active form is found extracellularly as a disulfide linked homodimer. This protein plays important roles in innate immunity, cancer and inflammatory diseases, including systemic lupus erythematosus, arthritis, atherosclerosis and obesity. In several conditions, activation of macrophages involves a CSF1 autocrine loop. In addition, secreted and cell-surface isoforms of CSF1 can have differential effects in inflammation and immunity. Recombinant human CSF1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human M-CSF stimulates cell proliferation of the M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range ≤3 ng/mL.

Product Name Alternative

Macrophage colony-stimulating factor 1, CSF1, M-CSF, MCSF, Lanimostim, ATGP3269

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range ≤3 ng/mL.

Molecular Weight

26.1 kDa (231aa)

Additionnal Information

M-CSF, ATGP3565-10 µg, ATGP3565-20 µg, ATGP3565-50 µg, ATGP3565-100 µg, ATGP3565-250 µg, ATGP3565-500 µg, ATGP3565-1 mg, ATGP3565-10, ATGP3565-20, ATGP3565-50, ATGP3565-100, ATGP3565-250, ATGP3565-500, ATGP3565-1

References & Citations

Chitu V., et al. (2006) Curr Opin Immunol. 18 (1) :39-48.; ; Eda H., et al. (2010) Cytokine. 52 (3) :215-220.; ; Tian F., et al. (2013) Oncol. Res. 20 (8) :341-350.