Recombinant human PHPT1 protein
PHPT1 is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. Recombinant human PHPT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Specific activity is >120 units/mg, and is defined as the amount of enzyme that hydrolyze 1nmol f p-nitrophenyl phosphate per minute at pH 7.5 at 37C.
Product Name Alternative
14 kDa phosphohistidine phosphatas, bA216L13.10, CGI-202, DKFZp564M173, HSPC141, PHP14, RP11-216L13.10
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
Enzyme Activity, SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is >120unit/mg, and is defined as the amount of enzyme that hydrolyze 1nmol f p-nitrophenyl phosphate per minute at pH 7.5 at 37C.
Molecular Weight
15.9 kDa (145aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Srivastava S., et al. (2008) Proc Natl Acad Sci U S A. 105 (38) :14442-6.; ; van Vlierberghe P., et al. (2006) Leukemia. 20 (7) :1245-53.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 2mM DTT, 10% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_054891
Uniprot Accession Number
Q9NRX4
Species
Human
AA Sequence
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