Recombinant human SRSF1 protein

SRSF1, also known as Serine/arginine-rich splicing factor 1, is a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. This protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. In addition to being involved in the splicing process, it also mediates post-splicing activities, such as mRNA nuclear export and translation. Recombinant human SRSF1 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Serine/arginine-rich splicing factor 1, SRSF1, ASF, SF2, SF2p33, SFRS1, SRp30a

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford Assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

28.5 kDa (254aa)

Additionnal Information

SRSF1, Serine/arginine-rich splicing factor 1, SRSF1, ASF, SF2, SF2p33, SFRS1, SRp30a, ATGP3558-10 µg, ATGP3558-20 µg, ATGP3558-50 µg, ATGP3558-100 µg, ATGP3558-250 µg, ATGP3558-500 µg, ATGP3558-1 mg, ATGP3558-10, ATGP3558-20, ATGP3558-50, ATGP3558-100, ATGP3558-250, ATGP3558-500, ATGP3558-1

References & Citations

Xue F., et al. (2015) PLoS One. 10: e0115354.; ; Goncalves V., et al. (2014) RNA. 20:474-482.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM KCl, 1mM DTT, 0.2mM EDTA, 40% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_008855

Uniprot Accession Number

Q07955

Species

Human

N Terminal Sequence

Met1

AA Sequence

MSGGGVIRGP AGNNDCRIYV GNLPPDIRTK DIEDVFYKYG AIRDIDLKNR RGGPPFAFVE FEDPRDAEDA VYGRDGYDYD GYRLRVEFPR SGRGTGRGGG GGGGGGAPRG RYGPPSRRSE NRVVVSGLPP SGSWQDLKDH MREAGDVCYA DVYRDGTGVV EFVRKEDMTY AVRKLDNTKF RSHEGETAYI RVKVDGPRSP SYGRSRSRSR SRSRSRSRSN SRSRSYSPRR SRGSPRYSPR HSRSRSRTHH HHHH