Recombinant human 2B4/CD244 protein
CD244, also known as natural killer cell receptor 2B4 isoform 1, is a natural killer and T cell immunoglobulin superfamily surface protein and ligand of CD48. It is a cell surface glycoprotein related to CD2 and implicated in the regulation of natural killer and T lymphocyte function. It is expressed on all human NK cells, a subpopulation of T cells, basophils and monocytes. It activates NK cell mediated cytotoxicity, induces secretion of IFN-gamma and matrix metalloproteinases, and NK cell invasiveness. Recombinant human CD244, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human CD48/SLAMF2 (CAT# ATGP3642) is coated at 2 µg/mL (100 ul/well) can bind Human CD244. The ED50 range ≤ 0.1 µg/mL.
Product Name Alternative
CD244 molecule natural killer cell receptor 2B4, NAIL, NKR2B4, Nmrk, SLAMF4
Expression System
Baculovirus
Antigen Species
Human
Tag
HIgG-His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human CD48/SLAMF2 (CAT# ATGP3642) . The ED50 range ≤ 0.1 µg/mL.
Molecular Weight
50.2 kDa (448aa)
Additionnal Information
References & Citations
Brown MH., et al. (1998) J Exp Med. 188:2083-2090.; ; Chuang SS., et al. (2001) J Immunol. 167:6210-6216.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_057466.1
Uniprot Accession Number
Q9BZW8
Species
Human
N Terminal Sequence
Ala
AA Sequence
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