Recombinant mouse eIF-4E/EIF4E protein
Eif4e, also known as Eukaryotic translation initiation factor 4E, modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. This protein recognizes and binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, thereby mediating the initiation of translation. It facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Recombinant mouse Eif4e protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Eukaryotic translation initiation factor 4E, EG668879, eIF-4E, Eif4e-ps, If4e, eIF-4F 25 kDa subunit
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Purity
> 95% by SDS-PAGE
Molecular Weight
27.6 kDa (241aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Piron F., et al. (2010) PLoS One. 5 (6) :e11313.; ; Cao R., et al. (2015) Nat neurosci. 18 (6) :855-62.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl, 0.1mM PMSF
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_031943.3
Uniprot Accession Number
P63073
Species
Mouse
AA Sequence
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