Recombinant canine IL-2 (C147S) protein
IL2, also known as Interleukin2, is an immunoregulatory lymphokine produced by T-cell in response to antigenic or mitogenic stimulation IL2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated kill cells, monocytres, macrophages and oligodendrocytes. Recombinant canine IL2 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Canine IL-2 stimulates cell cytotoxicity in the CTLL-2 mouse cytotoxic T cell. The ED50 range ≤ 0.3 ng/mL.
Product Name Alternative
Interleukin-2, IL-2, T-cell growth factor, TCGF
Expression System
E.coli
Antigen Species
Canine
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 range ≤ 0.3 ng/mL.
Molecular Weight
18.1 kDa (160aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Nedoszytko B., et al. (2016) Postepy Dermatol Alerqol. 33 (6) : 429-434.; ; Michael HT., et al. (2013) Vet Immunol Immunopathol. 155 (3) : 211-7.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4)
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_001003305
Uniprot Accession Number
Q29416
Species
Canine
AA Sequence
APITS SSTKETEQQM EQLLLDLQLL LNGVNNYENP QLSRMLTFKF YTPKKATEFT HLQCLAEELK NLEEVLGLPQ SKNVHLTDTK ELISNMNVTL LKLKGSETSY NCEYDDETAT ITEFLNKWIT FSQSIFSTLT
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