Recombinant human Granzyme K protein

GZMK, as known as Granzyme K, is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes. Recombinant human GZMK, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Granzyme K, GZMK, TRYP2, GZMK, PRSS, tryptase II, Fragmentin-3, Granzyme-3, NK-tryptase-2, NK-Tryp-2

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

26.9 kDa (247aa)

Additionnal Information

GZMK, ATGP3539-10 µg, ATGP3539-20 µg, ATGP3539-50 µg, ATGP3539-100 µg, ATGP3539-250 µg, ATGP3539-500 µg, ATGP3539-1 mg, ATGP3539-10, ATGP3539-20, ATGP3539-50, ATGP3539-100, ATGP3539-250, ATGP3539-500, ATGP3539-1

References & Citations

Jiang W., et al, (2011) J. Immunol. 187:781-790.; ; Wensink AC., et al, (2014) Proc. Natl. Acad. Sci. U.S.A. 111:5974-5979.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol, 1mM DTT

Scientific Category

Apoptosis

NCBI Accession Number

NP_002095.1

Uniprot Accession Number

P49863

Species

Human

N Terminal Sequence

Ala

AA Sequence

IIGGKEV SPHSRPFMAS IQYGGHHVCG GVLIDPQWVL TAAHCQYRFT KGQSPTVVLG AHSLSKNEAS KQTLEIKKFI PFSRVTSDPQ SNDIMLVKLQ TAAKLNKHVK MLHIRSKTSL RSGTKCKVTG WGATDPDSLR PSDTLREVTV TVLSRKLCNS QSYYNGDPFI TKDMVCAGDA KGQKDSCKGD SGGPLICKGV FHAIVSGGHE CGVATKPGIY TLLTKKYQTW IKSNLVPPHT N