Recombinant mouse ALT1/GPT1 protein

Gpt, also known as alanine aminotransferases 1, catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. This protein plays a key role in the intermediary metabolism of glucose and amino acids. It is widely used as an index of liver integrity or hepatocellular damage in clinical settings. Recombinant mouse Gpt protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Specifications

Specific activity is > 40units/mg, and is defined as the amount of enzyme that convert 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C

Product Name Alternative

Alanine aminotransferase 1, ALT, ALT1, Gpt-1, Gpt1

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 100unit/mg, and is defined as the amount of enzyme that convert 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C

Molecular Weight

57.5 kDa (519aa) confirmed by MALDI-TOF

Additionnal Information

ALT1, GPT1, ATGP3530-10 µg, ATGP3530-20 µg, ATGP3530-50 µg, ATGP3530-100 µg, ATGP3530-250 µg, ATGP3530-500 µg, ATGP3530-1 mg, ATGP3530-10, ATGP3530-20, ATGP3530-50, ATGP3530-100, ATGP3530-250, ATGP3530-500, ATGP3530-1

References & Citations

Sohocki MM. et al. (1997) Genomics. 40 (2) :247-52.; ; Matthews CC. et al. (2003) Brain Res. 978 (1-2) :59-64.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 1mM DTT, 10% glycerol

Scientific Category

Metabolism

NCBI Accession Number

NP_877957.1

Uniprot Accession Number

Q8QZR5

Species

Mouse

AA Sequence

MASQRND RIQASRNGLK GKVLTLDTMN PCVRRVEYAV RGPIVQRALE LEQELRQGVK KPFTEVIRAN IGDAQAMGQR PITFFRQVLA LCVYPNLLSS PDFPEDAKRR AERILQACGG HSLGAYSISS GIQPIREDVA QYIERRDGGI PADPNNIFLS TGASDAIVTM LKLLVAGEGR ARTGVLIPIP QYPLYSAALA ELDAVQVDYY LDEERAWALD IAELRRALCQ ARDRCCPRVL CVINPGNPTG QVQTRECIEA VIRFAFEEGL FLMADEVYQD NVYAEGSQFH SFKKVLTEMG PPYATQQELA SFHSVSKGYM GECGFRGGYV EVVNMDAEVQ KQMAKLMSVR LCPPVPGQAL MGMVVSPPTP SEPSFKQFQA ERQEVLAELA AKAKLTEQVF NEAPGIRCNP VQGAMYSFPQ IQLPLKAVQR AQDLGLAPDM FFCLCLLEET GICVVPGSGF GQQEGTYHFR MTILPPMEKL RVLLEKLRHF HAKFTHEYS