Recombinant human Osteoactivin/GPNMB protein

GPNMB, also known as glycoprotein nmb, is a transmembrane glycoprotein that is expressed in numerous cells, including osteoclasts, macrophages, dendritic cells, and tumor cells. It shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. This protein participates in bone mineralization, and functions as a negative regulator of inflammation in macrophages. It may be involved in growth delay and reduction of metastatic potential. Recombinant human GPNMB protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Transmembrane glycoprotein NMB, Glycoprotein NMB, Glycoprotein nonmetastatic melanoma protein B, Hematopoietic growth factor inducible neurokinin-1, HGFIN

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by BCA assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

51.8 kDa (462aa)

Additionnal Information

GPNMB, Glycoprotein nmb, GPNMB, NMB, HGFIN, ATGP3523-10 µg, ATGP3523-20 µg, ATGP3523-50 µg, ATGP3523-100 µg, ATGP3523-250 µg, ATGP3523-500 µg, ATGP3523-1 mg, ATGP3523-10, ATGP3523-20, ATGP3523-50, ATGP3523-100, ATGP3523-250, ATGP3523-500, ATGP3523-1

References & Citations

Huang JJ., et al. (2012) Brain Behav. 2: 85-96.; ; Kumagai K., et al. (2015) PLoS One. 10: e0143413.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_002501.1

Uniprot Accession Number

Q14956

Species

Human

N Terminal Sequence

Ala

AA Sequence

AKRFHDV LGNERPSAYM REHNQLNGWS SDENDWNEKL YPVWKRGDMR WKNSWKGGRV QAVLTSDSPA LVGSNITFAV NLIFPRCQKE DANGNIVYEK NCRNEAGLSA DPYVYNWTAW SEDSDGENGT GQSHHNVFPD GKPFPHHPGW RRWNFIYVFH TLGQYFQKLG RCSVRVSVNT ANVTLGPQLM EVTVYRRHGR AYVPIAQVKD VYVVTDQIPV FVTMFQKNDR NSSDETFLKD LPIMFDVLIH DPSHFLNYST INYKWSFGDN TGLFVSTNHT VNHTYVLNGT FSLNLTVKAA APGPCPPPPP PPRPSKPTPS LGPAGDNPLE LSRIPDENCQ INRYGHFQAT ITIVEGILEV NIIQMTDVLM PVPWPESSLI DFVVTCQGSI PTEVCTIISD PTCEITQNTV CSPVDVDEMC LLTVRRTFNG SGTYCVNLTL GDDTSLALTS TLISVP