Recombinant mouse 15-PGDH/HPGD protein

Hpgd, also known as Hydroxyprostaglandin dehydrogenase, is a member of the short-chain dehydrogenases/reductases family. It metabolizes prostaglandins (PGs) to render them. Prostaglandins (PGs) play a crucial role in mediating parturition events, and their synthesis and metabolism are regulated by PGH synthase and 15-hydroxy-PG dehydrogenase (PGDH), respectively. Hpgd, a COX-2 oncogene antagonist, is a TGF-beta-induced suppressor of human gastrointestinal cancers. Recombinant mouse Hpgd protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

15-hydroxyprostaglandin dehydrogenase [NAD+], 15-PGDH, PGDH, PGDH1, SDR36C1, Prostaglandin dehydrogenase 1

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Purity

> 95% by SDS-PAGE

Molecular Weight

31.6 kDa (292aa)

Additionnal Information

15 Hydroxyprostaglandin dehydrogenase, 15 Hydroxyprostaglandin dehydrogenase [NAD (+) ], 15 hydroxyprostaglandin dehydrogenase [NAD+], 15 PGDH, 15-Hydroxyprostaglandin dehydrogenase, 15-Hydroxyprostaglandin dehydrogenase [NAD (+) ], 15-hydroxyprostaglandin dehydrogenase [NAD+], 15PGDH, 15-PGDH, AV026552, HPGD, PGDH, PGDH1, Prostaglandin dehydrogenase 1, SDR36C1, ATGP3517-10 µg, ATGP3517-20 µg, ATGP3517-50 µg, ATGP3517-100 µg, ATGP3517-250 µg, ATGP3517-500 µg, ATGP3517-1 mg, ATGP3517-010, ATGP3517-020, ATGP3517-050, ATGP3517-100, ATGP3517-250, ATGP3517-500, ATGP3517-01M

References & Citations

Patel FA. et al., (2003) J Clin Endocrinol Metab. 88 (6) :2922-33.; ; McKeown KJ. et al., (2003) J Clin Endocrinol Metab. 88 (4) :1737-41.; ; Yan M. et al., (2004) Proc Natl Acad Sci USA. 101 (50) :17468-73.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_032304.2

Uniprot Accession Number

Q8VCC1

Species

Mouse

AA Sequence

MHVNGKV ALVTGAAQGI GKAFAEALLL HGAKVALVDW NLEAGVKCKA ALDEQFEPQK TLFVQCDVAD QKQLRDTFRK VVDHFGRLDI LVNNAGVNNE KNWEQTLQIN LVSVISGTYL GLDYMSKQNG GEGGIIINMS SLAGLMPVAQ QPVYCASKHG IIGFTRSAAM AANLMKSGVR LNVICPGFVD TPILESIEKE ENMGQYIEYK DQIKAMMKFY GVLHPSTIAN GLINLIEDDA LNGAIMKITA SKGIHFQDYD ISPLLVKAPL TS