Recombinant human TRACP/PAP/ACP5 protein

ACP5, also known as tartrate-resistant acid phosphatase type 5, is involved in osteopontin and bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. Recombinant human ACP5 was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is >10,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C

Product Name Alternative

Tartrate-resistant acid phosphatase type 5, SPENCDI, TRAP, HPAP, TRACP5a, TRACP5b, TrATPase

Expression System

Baculovirus

Antigen Species

Human

Tag

Non-Tagged

Applications

Enzyme Activity, SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is >10,000unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C

Molecular Weight

34.3 kDa (304aa)

Additionnal Information

TRACP, PAP, ACP5, Tartrate-resistant acid phosphatase type 5, SPENCDI, TRAP, HPAP, TRACP5a, TRACP5b, TrATPase, ATGP3514-10 µg, ATGP3514-20 µg, ATGP3514-50 µg, ATGP3514-100 µg, ATGP3514-250 µg, ATGP3514-500 µg, ATGP3514-1 mg, ATGP3514-10, ATGP3514-20, ATGP3514-50, ATGP3514-100, ATGP3514-250, ATGP3514-500, ATGP3514-1

References & Citations

Janckila A.J. et al., (1992) Clin. Biochem. 25:437-443.; ; Hayman A.R. et al., (1991) Biochem. J. 277:631-634.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001104505

Uniprot Accession Number

P13686

Species

Human

AA Sequence

ATPALRFVAV GDWGGVPNAP FHTAREMANA KEIARTVQIL GADFILSLGD NFYFTGVQDI NDKRFQETFE DVFSDRSLRK VPWYVLAGNH DHLGNVSAQI AYSKISKRWN FPSPFYRLHF KIPQTNVSVA IFMLDTVTLC GNSDDFLSQQ PERPRDVKLA RTQLSWLKKQ LAAAREDYVL VAGHYPVWSI AEHGPTHCLV KQLRPLLATY GVTAYLCGHD HNLQYLQDEN GVGYVLSGAG NFMDPSKRHQ RKVPNGYLRF HYGTEDSLGG FAYVEISSKE MTVTYIEASG KSLFKTRLPR RARP