Recombinant human IL-5 protein
IL5, also known as interleukin-5, is a member of the hematopoietin receptor superfamily and is comprised of a cytokine-specific alpha chain and the common beta chain that is shared among these cytokines for signaling. It related hematopoietic cytokines that are important for allergic inflammation. It is a lineage-specific cytokine for eosinophilpoiesis and plays an important part in diseases associated with increased eosinophils, such as asthma. Recombinant human IL5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤1.5 ng/mL.
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤1.5 ng/mL.
Molecular Weight
14.2 kDa (124aa)
Additionnal Information
References & Citations
Martinez-Moczygemba M., et al. (2003) J Allergy Clin Immunol. 112:653-665.; ; Milburn MV., et al. (1993) Nature. 363:172-176.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_000870
Uniprot Accession Number
P05113
Species
Human
N Terminal Sequence
Ala
AA Sequence
IPTEIPT SALVKETLAL LSTHRTLLIA NETLRIPVPV HKNHQLCTEE IFQGIGTLES QTVQGGTVER LFKNLSLIKK YIDGQKKKCG EERRRVNQFL DYLQEFLGVM NTEWIIES
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