Recombinant mouse 5′-Nucleotidase/CD73 protein

Nt5e, also known as 5-nucleotidase, is the main enzyme responsible for conversion of AMP into the immunosuppressive molecule adenosine. It was demonstrated to play a direct role in tumor progression including regulation of tumor vascularization. It is of general functional importance for the metabolism of nucleotides at the ventricular surface of the retina as well as the ventricles of the brain, a feature that is maintained throughout development. Recombinant mouse Nt5e, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 8,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of Adenosine 5-monophosphate to phosphate per minute per minute at pH 7.5 at 25C.

Product Name Alternative

CD73, 5-NT, 5'-nucleotidase, 5'-nucleotidase ecto, AI447961, E5NT, ecto, eN, eNT, NT, NT5, Nt5e, NTE, 2210401F01Rik

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 8,000pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of Adenosine 5-monophosphate to phosphate per minute per minute at pH 7.5 at 25C.

Molecular Weight

59.1 kDa (531aa)

Additionnal Information

Purine 5-Prime-Nucleotidase, NTE, NT5E, NT5, NT, eNT, eN, ecto-5'-nucleotidase, ecto, CD73, CALJA, AI447961, 5-nucleotidase ecto, 5'-nucleotidase ecto, 5-nucleotidase, 5'-nucleotidase, 5-NT, 5'-NT, 5' nucleotidase ecto, 5 nucleotidase ecto, 5' nucleotidase, 5 nucleotidase, 5' NT, 2210401F01Rik, ATGP3509-10 µg, ATGP3509-20 µg, ATGP3509-50 µg, ATGP3509-100 µg, ATGP3509-250 µg, ATGP3509-500 µg, ATGP3509-1 mg, ATGP3509-010, ATGP3509-020, ATGP3509-050, ATGP3509-100, ATGP3509-250, ATGP3509-500, ATGP3509-01M