Recombinant human t-Plasminogen Activator/PLAT protein

PLAT, also known as plasminogen activator, tissue type, is serine protease secreted by many cell types that participate in biological processes, such as tissue restructuring, cell migration, and tumor metastasis. It used to dissolve coronary fibrin clots that are the proximal causes of acute myocardial infarction. It plays a crucial role in regulating blood fibrinolysis, in maintaining the homeostasis of extracellular matrix and in modulating the post-translational activation of growth factors. Recombinant human PLAT, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Plasminogen activator, tissue type, PLAT, T-PA, TPA

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

29.2 kDa (261aa)

Additionnal Information

T-Plasminogen Activator, tPA, Plasminogen activator, tissue type, PLAT, T-PA, TPA, ATGP3504-10 µg, ATGP3504-20 µg, ATGP3504-50 µg, ATGP3504-100 µg, ATGP3504-250 µg, ATGP3504-500 µg, ATGP3504-1 mg, ATGP3504-10, ATGP3504-20, ATGP3504-50, ATGP3504-100, ATGP3504-250, ATGP3504-500, ATGP3504-1

References & Citations

Orth K., et al. (1992) Proc Natl Acad Sci U S A. 89:7422-7426.; ; Hu K., et al. (2008) Front Biosci. 13:5174-5186.

Other References

Hirayama S, et al. Triosephosphate isomerase of Streptococcus pneumoniae is released extracellularly by autolysis and binds to host plasminogen to promote its activation. (FEBS Open Bio. 2022) {https://pubmed.ncbi.nlm.nih.gov/35298875/}; ; Hirayama S, et al. C-Terminal Lysine Residue of Pneumococcal Triosephosphate Isomerase Contributes to Its Binding to Host Plasminogen. (Microorganisms. 2023) {https://pubmed.ncbi.nlm.nih.gov/37317172/}; ; Yasui Y, et al. The Pneumococcal Protein SufC Binds to Host Plasminogen and Promotes Its Conversion into Plasmin. (Microorganisms. 2023) {https://pubmed.ncbi.nlm.nih.gov/38138113/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 50 mM MES buffer (pH 5.0) containing 5mM CaCl2, 1mM DTT, 0.5M NaCl, 30% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_000921.1

Uniprot Accession Number

P00750

Species

Human

N Terminal Sequence

Ala

AA Sequence

IKGGLFA DIASHPWQAA IFAKHRRSPG ERFLCGGILI SSCWILSAAH CFQERFPPHH LTVILGRTYR VVPGEEEQKF EVEKYIVHKE FDDDTYDNDI ALLQLKSDSS RCAQESSVVR TVCLPPADLQ LPDWTECELS GYGKHEALSP FYSERLKEAH VRLYPSSRCT SQHLLNRTVT DNMLCAGDTR SGGPQANLHD ACQGDSGGPL VCLNDGRMTL VGIISWGLGC GQKDVPGVYT KVTNYLDWIR DNMRP