Recombinant human Carnosine Dipeptidase 1/CNDP1 protein
CNDP1, also known as beta-Ala-His dipeptidase, belongs to the peptidase M20A family. The shortest allelic form (CNDP1 Mannheim) was more common in the absence of nephropathy and was associated with lower serum carnosinase levels. Carnosine inhibited the increased production of fibronectin and collagen type VI in podocytes and the increased production of TGF-beta in mesangial cells. Diabetic patients with the CNDP1 Mannheim variant are less susceptible for nephropathy. Carnosine protects against the adverse effects of high glucose levels on renal cells. Recombinant human CNDP1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Specific activity is >3,000 pmol/min/ug, and as measured by the hydrolysis of carnosine per minute at pH6.8 at 25C.
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is >3,000pmol/min/ug, and as measured by the hydrolysis of carnosine per minute at pH6.8 at 25C.
Molecular Weight
54.9 kDa (489aa)
Additionnal Information
References & Citations
Kurashige M., et al. (2013) PLoS ONE 8 (1) :E54064.; ; Ahluwalia TS., et al. (2011) Diabetologia 54 (9) :2295-2302.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_116038.4
Uniprot Accession Number
Q96KN2
Species
Human
N Terminal Sequence
Ser27
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