Recombinant human Carnosine Dipeptidase 1/CNDP1 protein

CNDP1, also known as beta-Ala-His dipeptidase, belongs to the peptidase M20A family. The shortest allelic form (CNDP1 Mannheim) was more common in the absence of nephropathy and was associated with lower serum carnosinase levels. Carnosine inhibited the increased production of fibronectin and collagen type VI in podocytes and the increased production of TGF-beta in mesangial cells. Diabetic patients with the CNDP1 Mannheim variant are less susceptible for nephropathy. Carnosine protects against the adverse effects of high glucose levels on renal cells. Recombinant human CNDP1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is >3,000 pmol/min/ug, and as measured by the hydrolysis of carnosine per minute at pH6.8 at 25C.

Product Name Alternative

Beta-Ala-His dipeptidase, CNDP dipeptidase 1, Carnosine dipeptidase 1, Glutamate carboxypeptidase-like protein 2, Serum carnosinase, CN1, CPGL2, Carnosinase 1

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is >3,000pmol/min/ug, and as measured by the hydrolysis of carnosine per minute at pH6.8 at 25C.

Molecular Weight

54.9 kDa (489aa)

Additionnal Information

Beta-Ala-His dipeptidase, Carnosine dipeptidase 1, Carnosine Dipeptidase1, CN1, CNDP dipeptidase 1, CNDP1, CPGL2, Glutamate carboxypeptidase-like protein 2, HST2308, ATGP3503-10 µg, ATGP3503-20 µg, ATGP3503-50 µg, ATGP3503-100 µg, ATGP3503-250 µg, ATGP3503-500 µg, ATGP3503-1 mg, ATGP3503-010, ATGP3503-020, ATGP3503-050, ATGP3503-100, ATGP3503-250, ATGP3503-500, ATGP3503-01M