Recombinant human TDP-43/TARDBP protein

TARDBP, as known as TAR DNA binding protein 43, was originally identified as a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. It has also been identified in individuals diagnosed with chronic traumatic encephalopathy, a condition that often mimics ALS and that has been associated with athletes who have experienced multiple concussions and other types of head injury. It has been shown to bind both DNA and RNA and have multiple functions in transcriptional repression, pre-mRNA splicing and translational regulation. Recombinant human TARDBP protein was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

TAR DNA binding protein 43, ALS10, TDP-43

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

44.7 kDa (414aa)

Additionnal Information

TDP43, TARDBP, TAR DNA binding protein 43, ALS10, TDP-43, ATGP3502-10 µg, ATGP3502-20 µg, ATGP3502-50 µg, ATGP3502-100 µg, ATGP3502-250 µg, ATGP3502-500 µg, ATGP3502-1 mg, ATGP3502-10, ATGP3502-20, ATGP3502-50, ATGP3502-100, ATGP3502-250, ATGP3502-500, ATGP3502-1

References & Citations

Ou SH., et al. (1995) J Virol. 69 (6) :3584-96.

Other References

Ko VI, et al, CK1δ/ε kinases regulate TDP-43 phosphorylation and are therapeutic targets for ALS-related TDP-43 hyperphosphorylation. (Neurobiol Dis. 2024) {https://pubmed.ncbi.nlm.nih.gov/38677657/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT, 1mM EDTA, 10% glycerol

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_031401.1

Uniprot Accession Number

Q13148

Species

Human

AA Sequence

MSEYIRVTED ENDEPIEIPS EDDGTVLLST VTAQFPGACG LRYRNPVSQC MRGVRLVEGI LHAPDAGWGN LVYVVNYPKD NKRKMDETDA SSAVKVKRAV QKTSDLIVLG LPWKTTEQDL KEYFSTFGEV LMVQVKKDLK TGHSKGFGFV RFTEYETQVK VMSQRHMIDG RWCDCKLPNS KQSQDEPLRS RKVFVGRCTE DMTEDELREF FSQYGDVMDV FIPKPFRAFA FVTFADDQIA QSLCGEDLII KGISVHISNA EPKHNSNRQL ERSGRFGGNP GGFGNQGGFG NSRGGGAGLG NNQGSNMGGG MNFGAFSINP AMMAAAQAAL QSSWGMMGML ASQQNQSGPS GNNQNQGNMQ REPNQAFGSG NNSYSGSNSG AAIGWGSASN AGSGSGFNGG FGSSMDSKSS GWGM