Recombinant human Ephrin-B3 protein

EFNB3, also known as ephrin-B3, is a member of the Ephrin-B family of transmembrane ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. EFNB3 is expressed on oligodendrocytes and neurons in the hippocampus and along the midline of the spinal cord. It is up-regulated in glioma and promotes tumor cell invasion and migration. This protein acts as the midline barrier that prevents corticospinal tract projections from recrossing when they enter the spinal gray matter. Recombinant human EFNB3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ephrin-B3, EFL6, EPLG8, LERK8, EPH-related receptor transmembrane ligand ELK-L3, EPH-related receptor tyrosine kinase ligand 8

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

23 kDa (208aa)

Additionnal Information

Ephrin-B3, Ephrin-B3, EFNB3, EFL6, EPLG8, LERK8, ATGP3499-10 µg, ATGP3499-20 µg, ATGP3499-50 µg, ATGP3499-100 µg, ATGP3499-250 µg, ATGP3499-500 µg, ATGP3499-1 mg, ATGP3499-10, ATGP3499-20, ATGP3499-50, ATGP3499-100, ATGP3499-250, ATGP3499-500, ATGP3499-1

References & Citations

Miao H., et al. (2009) Int J Biochem Cell Biol. 41:762-770.; ; Kullander K., et al. (2001) Genes Dev. 15:877-888.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_001397

Uniprot Accession Number

Q15768

Species

Human

N Terminal Sequence

Ala

AA Sequence

LSLEPVY WNSANKRFQA EGGYVLYPQI GDRLDLLCPR ARPPGPHSSP NYEFYKLYLV GGAQGRRCEA PPAPNLLLTC DRPDLDLRFT IKFQEYSPNL WGHEFRSHHD YYIIATSDGT REGLESLQGG VCLTRGMKVL LRVGQSPRGG AVPRKPVSEM PMERDRGAAH SLEPGKENLP GDPTSNATSR GAEGPLPPPS MP