Recombinant human CD39L3/ENTPD3 protein

ENTPD3, also known as ectonucleoside triphosphate diphosphohydrolase 3, is an integral membrane glycoprotein with an extracellular active site. It contains 4 apyrase-conserved regions which is characteristic of NTPases. Recombinant human ENTPD3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 250,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze ATP per minute at pH 7.5 at 37C.

Product Name Alternative

Ectonucleoside triphosphate diphosphohydrolase 3, NTPDase 3, CD39 antigen-like 3, Ecto-ATP diphosphohydrolase 3, Ecto-ATPDase 3, Ecto-ATPase 3, Ecto-apyrase 3, HB6

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 250,000pmol/min/ug, and is defined as the amount of enzyme that hydrolyze ATP per minute at pH 7.5 at 37C.

Molecular Weight

50.7 kDa (451aa)

Additionnal Information

CD39L3, ENTPD3, Ectonucleoside triphosphate diphosphohydrolase 3, ENTPD3, CD39L3, HB6, NTPDase-3, ATGP3498-10 µg, ATGP3498-20 µg, ATGP3498-50 µg, ATGP3498-100 µg, ATGP3498-250 µg, ATGP3498-500 µg, ATGP3498-1 mg, ATGP3498-10, ATGP3498-20, ATGP3498-50, ATGP3498-100, ATGP3498-250, ATGP3498-500, ATGP3498-1

References & Citations

Lavoie EG., et al. (2004) Biochem. Pharmacol. 67:1917-1926.; ; Fausther M., et al. (2010) Am J Physiol Lung Cell Mol Physiol. 298:L804-818.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001239

Uniprot Accession Number

O75355

Species

Human

N Terminal Sequence

Ala

AA Sequence

ADLQIHKQEV LPPGLKYGIV LDAGSSRTTV YVYQWPAEKE NNTGVVSQTF KCSVKGSGIS SYGNNPQDVP RAFEECMQKV KGQVPSHLHG STPIHLGATA GMRLLRLQNE TAANEVLESI QSYFKSQPFD FRGAQIISGQ EEGVYGWITA NYLMGNFLEK NLWHMWVHPH GVETTGALDL GGASTQISFV AGEKMDLNTS DIMQVSLYGY VYTLYTHSFQ CYGRNEAEKK FLAMLLQNSP TKNHLTNPCY PRDYSISFTM GHVFDSLCTV DQRPESYNPN DVITFEGTGD PSLCKEKVAS IFDFKACHDQ ETCSFDGVYQ PKIKGPFVAF AGFYYTASAL NLSGSFSLDT FNSSTWNFCS QNWSQLPLLL PKFDEVYARS YCFSANYIYH LFVNGYKFTE ETWPQIHFEK EVGNSSIAWS LGYMLSLTNQ IPAESPLIRL PIEPPHHHHH H