Recombinant mouse IMPAD1 protein

IMPAD1, also known as inositol monophosphatase 3, may play a role in the formation of skeletal elements derived through endochondral ossification, possibly by clearing adenosine 3, 5-bisphosphate produced by Golgi sulfotransferases during glycosaminoglycan sulfation. Recombinant mouse IMPAD1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 5,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze Adenosine 3, 5-diphosphate per minute at pH 7.5 at 25C.

Product Name Alternative

Inositol monophosphatase 3, IMPAD1, 1110001C20Rik, AA408880, AI451589, AL022796, B230207P20, gPAPP, Jaws

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

Enzyme Activity, SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 5,000pmol/min/ug and is defined as the amount of enzyme that hydrolyze Adenosine 3, 5-diphosphate per minute at pH 7.5 at 25C.

Molecular Weight

36.2 kDa (332aa)

Additionnal Information

IMPAD1, Inositol monophosphatase 3, IMPAD1, 1110001C20Rik, AA408880, AI451589, AL022796, B230207P20, gPAPP, Jaws, ATGP3497-10 µg, ATGP3497-20 µg, ATGP3497-50 µg, ATGP3497-100 µg, ATGP3497-250 µg, ATGP3497-500 µg, ATGP3497-1 mg, ATGP3497-10, ATGP3497-20, ATGP3497-50, ATGP3497-100, ATGP3497-250, ATGP3497-500, ATGP3497-1

References & Citations

Strott CA. (2002) Endocr Rev. 23:703-732.; ; Frederick JP., et al. (2008) Proc Natl Acad Sci U S A. 105:11605-11612.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_808398

Uniprot Accession Number

Q80V26

Species

Mouse

N Terminal Sequence

Ala

AA Sequence

ADPGRFSLFG LGSEPAAGEA EVASDGGTVD LREMLAVAVL AAERGGDEVR RVRESNVLHE KSKGKTREGA DDKMTSGDVL SNRKMFYLLK TAFPNVQINT EEHVDASDKE VIVWNRKIPE DILKEIAAPK EVPAESVTVW IDPLDATQEY TEDLRKYVTT MVCVAVNGKP VLGVIHKPFS EYTAWAMVDG GSNVKARSSY NEKTPKIIVS RSHAGMVKQV ALQTFGNQTS IIPAGGAGYK VLALLDVPDM TQEKADLYIH VTYIKKWDIC AGNAILKALG GHMTTLNGEE ISYTGSDGIE GGLLASIRMN HQALVRKLPD LEKSGHHHHH HH