Recombinant rat Cyclophilin F/PPIF protein

Ppif also known as Peptidyl-prolyl cis-trans isomerase F, mitochondrial. Ppif accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and involved in regulation of the mitochondrial permeability transition pore (mPTP) . It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis the requirement of the PPIase activity for this function is debated. Recombinant rat Ppif was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 1,300 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Product Name Alternative

Peptidyl-prolyl cis-trans isomerase F mitochondrial, PPIase F, Cyclophilin D, CYPD, Cyclophilin F, Rotamase F

Expression System

E.coli

Antigen Species

Rat

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 1,300 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Molecular Weight

21.2Da (200aa) confirmed by MALDI-TOF

Additionnal Information

Cyclophilin D, Cyclophilin F, CYP D, CYPD, CYP-D, mitochondrial, Peptidyl-prolyl cis-trans isomerase F, Peptidyl-prolyl cis-trans isomerase F mitochondrial, PPIase, Ppif, ATGP3496-10 µg, ATGP3496-20 µg, ATGP3496-50 µg, ATGP3496-100 µg, ATGP3496-250 µg, ATGP3496-500 µg, ATGP3496-1 mg, ATGP3496-010, ATGP3496-020, ATGP3496-050, ATGP3496-100, ATGP3496-250, ATGP3496-500, ATGP3496-01M

References & Citations

Connern C.P., et al. (1992) Biochem. J. 284:381-385.; ; Lin DT., et al. (2002) J Biol Chem. 23:277 (34) :31134-41.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_758443

Uniprot Accession Number

P29117

Species

Rat

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSCSDGGAR GANSSSQNPL VYLDVGADGQ PLGRVVLELK ADVVPKTAEN FRALCTGEKG FGYKGSTFHR VIPAFMCQAG DFTNHNGTGG KSIYGSRFPD ENFTLKHVGP GVLSMANAGP NTNGSQFFIC TIKTDWLDGK HVVFGHVKEG MDVVKKIESF GSKSGKTSKK IVITDCGQLS