Recombinant rat Cyclophilin F/PPIF protein
Ppif also known as Peptidyl-prolyl cis-trans isomerase F, mitochondrial. Ppif accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and involved in regulation of the mitochondrial permeability transition pore (mPTP) . It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis the requirement of the PPIase activity for this function is debated. Recombinant rat Ppif was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
Peptidyl-prolyl cis-trans isomerase F mitochondrial, PPIase F, Cyclophilin D, CYPD, Cyclophilin F, Rotamase F
Expression System
E.coli
Antigen Species
Rat
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by absorbance at 280 nm)
Purity
> 90% by SDS-PAGE
Bioactivity
Molecular Weight
21.2Da (200aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Connern C.P., et al. (1992) Biochem. J. 284:381-385.; ; Lin DT., et al. (2002) J Biol Chem. 23:277 (34) :31134-41.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_758443
Uniprot Accession Number
P29117
Species
Rat
AA Sequence
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