Recombinant human Erythropoietin R/EPOR protein
EPOR, also known as Erythropoietin receptor, mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, it dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. So it may also activate the LYN tyrosine kinase. Recombinant human EPOR, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Measured by its ability to inhibit proliferation using TF-1 human erythroleukemic cells. The ED50 range ≤70 ng/mL with Human Erythropoietin.
Product Name Alternative
Erythropoietin receptor, EPO-R, EPOR
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured by its ability to inhibit proliferation using TF-1 human erythroleukemic cells. The ED50 range ≤70 ng/mL with Human Erythropoietin.
Molecular Weight
25.6 kDa (232aa)
Additionnal Information
References & Citations
Nakamura Y., et al. (1992) Science. 257:1138-1141.; ; Kirito K., et al. (2002) Blood. 99:102-110.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_000112
Uniprot Accession Number
P19235
Species
Human
N Terminal Sequence
Ala25
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