Recombinant human Apolipoprotein H/APOH protein

APOH, also known as Beta-2-glycoprotein 1, is a glycosylated member of the complement control superfamily of proteins. APOH binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. This protein may prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells. It has a complex involvement in agglutination, it appears to alter Adenosine diphosphate (ADP) mediated agglutination of platelets. Recombinant human APOH protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Beta-2-glycoprotein 1, APOH, BG, B2G1, B2GP1, Apolipoprotein H

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

37.3 kDa (335aa)

Additionnal Information

Apolipoprotein H, Beta-2-glycoprotein 1, APOH, BG, B2G1, B2GP1, Apolipoprotein H, ATGP3492-10 µg, ATGP3492-20 µg, ATGP3492-50 µg, ATGP3492-100 µg, ATGP3492-250 µg, ATGP3492-500 µg, ATGP3492-1 mg, ATGP3492-10, ATGP3492-20, ATGP3492-50, ATGP3492-100, ATGP3492-250, ATGP3492-500, ATGP3492-1

References & Citations

About F. et al., (2015) PLoS One. 10:e0145105. ; ; Laird ME. et al., (2014) J Hepatol. 61:770-776.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_000033

Uniprot Accession Number

P02749

Species

Human

N Terminal Sequence

Ala

AA Sequence

ADPGRTCPKP DDLPFSTVVP LKTFYEPGEE ITYSCKPGYV SRGGMRKFIC PLTGLWPINT LKCTPRVCPF AGILENGAVR YTTFEYPNTI SFSCNTGFYL NGADSAKCTE EGKWSPELPV CAPIICPPPS IPTFATLRVY KPSAGNNSLY RDTAVFECLP QHAMFGNDTI TCTTHGNWTK LPECREVKCP FPSRPDNGFV NYPAKPTLYY KDKATFGCHD GYSLDGPEEI ECTKLGNWSA MPSCKASCKV PVKKATVVYQ GERVKIQEKF KNGMLHGDKV SFFCKNKEKK CSYTEDAQCI DGTIEVPKCF KEHSSLAFWK TDASDVKPCH HHHHH