Recombinant mouse Cathepsin D protein
Ctsd, also known as cathepsin D, is peptidase belonging to the family of aspartic peptidases. It is intracellular catabolism in lysosomal compartments, other physiological effect include hormone and antigen processing. It plays an important role in alpha-synuclein degradation, and neuronal survival. It has also been shown to mediate apoptosis induced by various stimuli and p53-dependent tumor suppression. Recombinant mouse Ctsd, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
Cathepsin D, Ctsd, CatD, CD
Expression System
Baculovirus
Antigen Species
Mouse
Tag
His-Tag
Applications
Enzyme Activity, SDS-PAGE
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Specific activity is > 1,000pmol/min/ug in which one unit will convert 1.0pmole of Mca-PLGL-Dpa-AR-NH2 to MCA- Pro-Leu-OH per minute at pH 3.5 at 25C.
Molecular Weight
44 kDa (398aa)
Additionnal Information
References & Citations
Crabtree D., et al. (2013) Neurosci Lett. 538:3-8.; ; Tsukuba T., et al. (2000) Mol Cells. 10:601-611.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Neuroscience
NCBI Accession Number
NP_034113
Uniprot Accession Number
P18242
Species
Mouse
N Terminal Sequence
Ile21
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