Recombinant human TPI1 protein

TPI1 (Triosephosphate isomerase) belongs to the triosephosphate isomerase family. TPI1 catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) . TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Recombinant human TPI1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 3000 units/mg, in which one unit will convert 1.0 umole of D-glyceraldehyde-3-phosphate to dihydroxyacetone phosphate per minute at pH 7.5 at 25C.

Product Name Alternative

Triosephosphate isomerase, TPI

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

Enzyme Activity, SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 3000unit/mg, in which one unit will convert 1.0 umole of D-glyceraldehyde-3-phosphate to dihydroxyacetone phosphate per minute at pH 7.5 at 25C.

Molecular Weight

28.8 kDa (269aa) confirmed by MALDI-TOF

Additionnal Information

TPI1, triosephosphate isomerase, TPI, ATGP3489-10 µg, ATGP3489-20 µg, ATGP3489-50 µg, ATGP3489-100 µg, ATGP3489-250 µg, ATGP3489-500 µg, ATGP3489-1 mg, ATGP3489-10, ATGP3489-20, ATGP3489-50, ATGP3489-100, ATGP3489-250, ATGP3489-500, ATGP3489-1

References & Citations

Maquat L.E., et al. (1985) J. Biol. Chem. 260:3748-3753.; ; Rodriguez-Almazan C, et al. (2008) J Biol Chem. 22; ;283 (34) :23254-63.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_000356

Uniprot Accession Number

P60174

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MAPSRKFFVG GNWKMNGRKQ SLGELIGTLN AAKVPADTEV VCAPPTAYID FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDCGAT WVVLGHSERR HVFGESDELI GQKVAHALAE GLGVIACIGE KLDEREAGIT EKVVFEQTKV IADNVKDWSK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKSNVSDA VAQSTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ