Recombinant human t-Plasminogen Activator/PLAT protein
PLAT, as known as tissue-type plasminogen activator isoform 1, is a secreted serine protease synthesized by endothelial cells. This protein is secreted as a single chain polypeptide precursor which is cleaved in turn by plasmin. Also, it plays a role clinical medicine to treat stroke in cell migration and tissue remodeling. It is implicated in normal neural function and is suggested as a regulatory molecule in neurodegenerative diseases. Recombinant human PLAT, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Tissue-type plasminogen activator isoform 1, PLAT, T-PA, TPA
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
61.3 kDa (545aa)
Additionnal Information
References & Citations
Chevilley A., et al, (2015) Front Cell Neurosci. 9:415.; ; Lansley SM., et al, (2015) Am. J. Respir. Cell Mol. Biol. 53:105-112.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 50 mM MES buffer (pH 5.5) containing 40% glycerol, 5mM CaCl2, 1mM DTT, 0.5M NaCl
Scientific Category
Cardiovascular
NCBI Accession Number
NP_000921
Uniprot Accession Number
P00750
Species
Human
N Terminal Sequence
Gln24
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