Recombinant human ADH1C protein

ADH1C, also known as Alcohol dehydrogenase 1C, belongs to the zinc-containing alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. It is a monomorphic and predominant in fetal and infant livers, becoming less active in gestation and only weakly active during adulthood. Recombinant human ADH1C protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Alcohol dehydrogenase 1C, ADH1C, ADH3

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

40.6 kDa (381aa)

Additionnal Information

ADH1C, Alcohol dehydrogenase 1C, ADH1C, ADH3, ATGP3481-10 µg, ATGP3481-20 µg, ATGP3481-50 µg, ATGP3481-100 µg, ATGP3481-250 µg, ATGP3481-500 µg, ATGP3481-1 mg, ATGP3481-10, ATGP3481-20, ATGP3481-50, ATGP3481-100, ATGP3481-250, ATGP3481-500, ATGP3481-1

References & Citations

Jelski W., et al. (2007) Dig Dis Sci. 52:1513-1516.; ; Smith M., et al. (1973) Ann Hum Genet. 36:401-414.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_000660

Uniprot Accession Number

P00326

Species

Human

N Terminal Sequence

Met1

AA Sequence

MSTAGKVIKC KAAVLWELKK PFSIEEVEVA PPKAHEVRIK MVAAGICRSD EHVVSGNLVT PLPVILGHEA AGIVESVGEG VTTVKPGDKV IPLFTPQCGK CRICKNPESN YCLKNDLGNP RGTLQDGTRR FTCSGKPIHH FVGVSTFSQY TVVDENAVAK IDAASPLEKV CLIGCGFSTG YGSAVKVAKV TPGSTCAVFG LGGVGLSVVM GCKAAGAARI IAVDINKDKF AKAKELGATE CINPQDYKKP IQEVLKEMTD GGVDFSFEVI GRLDTMMASL LCCHEACGTS VIVGVPPDSQ NLSINPMLLL TGRTWKGAIF GGFKSKESVP KLVADFMAKK FSLDALITNI LPFEKINEGF DLLRSGKSIR TVLTFHHHHH H