Recombinant human CRLF2 protein

CRLF2, also known as cytokine receptor-like factor 2 isoform 1, is a receptor for thymic stromal lymphopoietin. It is proposed to signal through a heterodimeric receptor complex that consists of a new member of the hemopoietin family termed CRLF2 and the IL-7R alpha-chain. The protein induces phosphorylation of Stat3 and Stat5 also activate JAK2 pathways. CRLF2 and IL-7R alpha are principally coexpressed on monocytes and dendritic cell populations and to a much lesser extent of various lymphoid cells. Recombinant human CRLF2, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cytokine receptor-like factor 2 isoform 1 precursor, CRL2, CRLF2Y, TSLPR, Cytokine receptor-like factor 2 isoform 1, CRLF2, Cytokine receptor-like 2, ILXR, Thymic stromal lymphopoietin protein receptor, TSLP receptor

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

25.2 kDa (218aa)

Additionnal Information

TSLPR, TSLP R, Cytokine receptor-like factor 2 isoform 1 precursor, CRLF2Y, CRLF2, CRL2, ATGP3478-10 µg, ATGP3478-20 µg, ATGP3478-50 µg, ATGP3478-100 µg, ATGP3478-250 µg, ATGP3478-500 µg, ATGP3478-1 mg, ATGP3478-010, ATGP3478-020, ATGP3478-050, ATGP3478-100, ATGP3478-250, ATGP3478-500, ATGP3478-01M

References & Citations

Reche PA., et al. (2001) J Immunol. 167:336-343.; ; Chen IM., et al. (2012) Blood. 119:3512-3522.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_071431.2

Uniprot Accession Number

Q9HC73

Species

Human

N Terminal Sequence

Ala

AA Sequence

QGGAAEG VQIQIIYFNL ETVQVTWNAS KYSRTNLTFH YRFNGDEAYD QCTNYLLQEG HTSGCLLDAE QRDDILYFSI RNGTHPVFTA SRWMVYYLKP SSPKHVRFSW HQDAVTVTCS DLSYGDLLYE VQYRSPFDTE WQSKQENTCN VTIEGLDAEK CYSFWVRVKA MEDVYGPDTY PSDWSEVTCW QRGEIRDACA ETPTPPKPKL SK