Recombinant human DNAM-1/CD226 protein

CD226, also known as CD226 antigen isoform, is a member of the immunoglobulin superfamily containing 2 Ig-like domains of the V-set. It is an activating receptor expressed on NK cells and T cells and plays an important role in cytotoxicity of these cells against target cells. It mediates platelet and megakaryocytic cell adhesion to vascular endothelial cells. Recombinant human CD226, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

CD226 antigen isoform, CD226, DNAM-1, DNAM1, PTA1, TLiSA1

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

53.2 kDa (471aa)

Additionnal Information

DNAM1, CD226, CD226 antigen isoform, CD226, DNAM-1, DNAM1, PTA1, TLiSA1, ATGP3475-10 µg, ATGP3475-20 µg, ATGP3475-50 µg, ATGP3475-100 µg, ATGP3475-250 µg, ATGP3475-500 µg, ATGP3475-1 mg, ATGP3475-10, ATGP3475-20, ATGP3475-50, ATGP3475-100, ATGP3475-250, ATGP3475-500, ATGP3475-1

References & Citations

Vo AV., et al. (2016) Mol Immunol. 69:70-76.; ; Kojima H., et al. (2003) J Biol Chem. 278:36748-36753.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_006557

Uniprot Accession Number

Q15762

Species

Human

N Terminal Sequence

Ala

AA Sequence

ADPEEVLWHT SVPFAENMSL ECVYPSMGIL TQVEWFKIGT QQDSIAIFSP THGMVIRKPY AERVYFLNST MASNNMTLFF RNASEDDVGY YSCSLYTYPQ GTWQKVIQVV QSDSFEAAVP SNSHIVSEPG KNVTLTCQPQ MTWPVQAVRW EKIQPRQIDL LTYCNLVHGR NFTSKFPRQI VSNCSHGRWS VIVIPDVTVS DSGLYRCYLQ ASAGENETFV MRLTVAEGKT DNLEPKSCDK THTCPPCPAP ELLGGPSVFL FPPKPKDTLM ISRTPEVTCV VVDVSHEDPE VKFNWYVDGV EVHNAKTKPR EEQYNSTYRV VSVLTVLHQD WLNGKEYKCK VSNKALPAPI EKTISKAKGQ PREPQVYTLP PSRDELTKNQ VSLTCLVKGF YPSDIAVEWE SNGQPENNYK TTPPVLDSDG SFFLYSKLTV DKSRWQQGNV FSCSVMHEAL HNHYTQKSLS LSPGKHHHHH H