Recombinant human sFRP-4/SFRP4 protein

SFRP4, also known as secreted frizzled-related protein4, is a family of vertebrate proteins which contain homology to the ligand-binding domain of the Frizzled family of transmembrane receptors. It is expressed in brain, kidney, lung, ovary, prostate, mammary gland and endometrium. This protein act as soluble modulators of Wnt signaling, counteracting Wnt-induced effects at high concentrations and promoting them at lower concentrations. It is able to bind Wnt proteins and Frizzled receptors in the extracellular compartment. Recombinant human SFRP4, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Secreted frizzled-related protein 4, SFRP4, FRP-4, FRPHE, PYL, sFRP-4, Human endometrium Frizzled protein, FRZB-2

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

38.9 kDa (337aa)

Additionnal Information

FRP 4, FRP4, FRP-4, FRPHE, FRZB 2, FRZB2, FRZB-2, Human endometrium Frizzled protein, PYL, Secreted frizzled related protein 4, Secreted frizzled-related protein 4, SFRP 4, SFRP4, SFRP-4, ATGP3470-10 µg, ATGP3470-20 µg, ATGP3470-50 µg, ATGP3470-100 µg, ATGP3470-250 µg, ATGP3470-500 µg, ATGP3470-1 mg, ATGP3470-010, ATGP3470-020, ATGP3470-050, ATGP3470-100, ATGP3470-250, ATGP3470-500, ATGP3470-01M

References & Citations

Jones SE., et al. (2002) Bioessays 24:811-820.; ; Berndt T., et al. (2003) J Clin Invest. 112:785-794.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Stem cell

NCBI Accession Number

NP_003005

Uniprot Accession Number

Q6FHJ7

Species

Human

N Terminal Sequence

Ala

AA Sequence

ADPVRGAPCE AVRIPMCRHM PWNITRMPNH LHHSTQENAI LAIEQYEELV DVNCSAVLRF FLCAMYAPIC TLEFLHDPIK PCKSVCQRAR DDCEPLMKMY NHSWPESLAC DELPVYDRGV CISPEAIVTD LPEDVKWIDI TPDMMVQERP LDVDCKRLSP DRCKCKKVKP TLATYLSKNY SYVIHAKIKA VQRSGCNEVT TVVDVKEIFK SSSPIPRTQV PLITNSSCQC PHILPHQDVL IMCYEWRSRM MLLENCLVEK WRDQLSKRSI QWEERLQEQR RTVQDKKKTA GRTSRSNPPK PKGKPPAPKP ASPKKNIKTR SAQKRTNPKR VHHHHHH