Recombinant mouse Carboxylesterase 3/CES3 protein

Ces1d, also known as carboxylesterase 1D, is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds. It is the principle lipase of white adipose tissue fat cake extracts. Partially purified white adipose tissue Ces1d had lipase activity as well as lesser but detectable neutral cholesteryl ester hydrolase activity. The protein shows low catalytic efficiency for hydrolysis of CPT-11, a prodrugs for camptothecin used in cancer therapeutics. Recombinant mouse Ces1d, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 80,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to p-nitrophenol per minute at pH 7.5 at 37C

Product Name Alternative

Carboxylesterase 1D, Ces1d, Ces3, TGH

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 80,000pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of p-nitrophenyl acetate to p-nitrophenol per minute at pH 7.5 at 37C

Molecular Weight

60.9 kDa (555aa)

Additionnal Information

Carboxylesterase3, CES3, Carboxylesterase 1D, Ces1d, Ces3, TGH, ATGP3467-10 µg, ATGP3467-20 µg, ATGP3467-50 µg, ATGP3467-100 µg, ATGP3467-250 µg, ATGP3467-500 µg, ATGP3467-1 mg, ATGP3467-10, ATGP3467-20, ATGP3467-50, ATGP3467-100, ATGP3467-250, ATGP3467-500, ATGP3467-1