Recombinant mouse Carboxylesterase 3/CES3 protein
Ces1d, also known as carboxylesterase 1D, is a member of a large family of carboxylesterases that are responsible for the hydrolysis of ester and amide bonds. It is the principle lipase of white adipose tissue fat cake extracts. Partially purified white adipose tissue Ces1d had lipase activity as well as lesser but detectable neutral cholesteryl ester hydrolase activity. The protein shows low catalytic efficiency for hydrolysis of CPT-11, a prodrugs for camptothecin used in cancer therapeutics. Recombinant mouse Ces1d, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
Carboxylesterase 1D, Ces1d, Ces3, TGH
Expression System
Baculovirus
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Molecular Weight
60.9 kDa (555aa)
Additionnal Information
References & Citations
Soni KG., et al. (2004) J. Biol. Chem. 279:40683-40689.; ; Sanghani SP., et al. (2004) Drug Metab Dispos. 32:505-511.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_444430
Uniprot Accession Number
Q8VCT4
Species
Mouse
N Terminal Sequence
Tyr19
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