Recombinant human HPRT protein

Hypoxanthine-guanine phosphoribosyltransferase, also known as HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. The enzyme primarily functions to salvage purines from degraded DNA to renewed purine synthesis. In this role, it acts as a catalyst in the reaction between guanine and phosphoribosyl pyrophosphate to form GMP. Recombinant human HPRT1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 15 units/mg and is defined as the amount of enzyme that catalyze the formation of 1 umole of guanosine 5-monophosphate (GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37C.

Product Name Alternative

Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase, HPRT

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

Enzyme Activity, SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 15unit/mg and is defined as the amount of enzyme that catalyze the formation of 1 umole of guanosine 5-monophosphate (GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37C.

Molecular Weight

26.7 kDa (238aa) confirmed by MALDI-TOF

Additionnal Information

HPRT, Hypoxanthine-guanine phosphoribosyltransferase, HGPRT, HGPRTase, HPRT, ATGP3466-10 µg, ATGP3466-20 µg, ATGP3466-50 µg, ATGP3466-100 µg, ATGP3466-250 µg, ATGP3466-500 µg, ATGP3466-1 mg, ATGP3466-10, ATGP3466-20, ATGP3466-50, ATGP3466-100, ATGP3466-250, ATGP3466-500, ATGP3466-1

References & Citations

Hladnik U., et al. (2008) Arch Neurol. 65 (9) :1240-3.; ; Sculley DG., et al. (1992) Hum Genet. 90 (3) :195-207191.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing, 20% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_000185

Uniprot Accession Number

P00492

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MATRSPGVVI SDDEPGYDLD LFCIPNHYAE DLERVFIPHG LIMDRTERLA RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV RQYNPKMVKV ASLLVKRTPR SVGYKPDFVG FEIPDKFVVG YALDYNEYFR DLNHVCVISE TGKAKYKA