Recombinant human Iduronate 2-Sulfatase protein
IDS, as known as iduronate 2-sulfatase isoform, is a member of the highly conserved sulfatase family of enzymes that catalyze the hydrolysis of O-sulfate and N-salfate esters from a varity of substrates. This protein is required for the lysosomal degradation of the glycosaminoglycans (GAG) heparan sulfate and dermatan sulfate. It hydrolyzes the 2-sulfate group of the IDS units of the GAG. Recombinant human IDS, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Iduronate 2-sulfatase isoform, IDS, MPS2, SIDS
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
60.3 kDa (533aa)
Additionnal Information
References & Citations
Torres LC., et al, (2014) Clin. Immunol. 154:100-104.; ; Chistiakov DA., et al, (2014) J. Genet. Genomics. 41:197-203.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_000193
Uniprot Accession Number
P22304
Species
Human
N Terminal Sequence
Ser26
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