Recombinant mouse Arylsulfatase A/ARSA protein

Arsa, also known as arylsulfatase A, is a prototype member of the sulfatase family. It is an enzyme that catalyzes the degradation of sulfatides, a glycosphingolipid found in many tissues, but predominantly in myelin and kidney. The serum level of its might be helpful in diagnosis of lung and central nervous system cancer. Recombinant mouse Arsa, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Arylsulfatase A, Arsa, As-2, AS-A, As2, ASA, AW212749, TISP73, Cerebroside-sulfatase

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

53.2 kDa (498aa)

Additionnal Information

Arsa, Arylsulfatase A, Arsa, As-2, AS-A, As2, ASA, AW212749, TISP73, ATGP3458-10 µg, ATGP3458-20 µg, ATGP3458-50 µg, ATGP3458-100 µg, ATGP3458-250 µg, ATGP3458-500 µg, ATGP3458-1 mg, ATGP3458-10, ATGP3458-20, ATGP3458-50, ATGP3458-100, ATGP3458-250, ATGP3458-500, ATGP3458-1

References & Citations

Lukatela G., et al. (1998) Biochemistry. 37:3654-3664.; ; Jean S., et al. (2006) Alcohol Clin Exp Res. 30:1950-1955.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_033843

Uniprot Accession Number

P50428

Species

Mouse

N Terminal Sequence

Ala

AA Sequence

ADPSPPNILL IFADDLGYGD LGSYGHPSST TPNLDQLAEG GLRFTDFYVP VSLCTPSRAA LLTGRLPVRS GMYPGVLGPS SQGGLPLEEV TLAEVLAARG YLTGMAGKWH LGVGPEGAFL PPHQGFHRFL GIPYSHDQGP CQNLTCFPPD IPCKGGCDQG LVPIPLLANL TVEAQPPWLP GLEARYVSFS RDLMADAQRQ GRPFFLYYAS HHTHYPQFSG QSFTKRSGRG PFGDSLMELD GAVGALMTTV GDLGLLEETL VIFTADNGPE LMRMSNGGCS GLLRCGKGTT FEGGVREPAL VYWPGHITPG VTHELASSLD LLPTLAALTG APLPNVTLDG VDISPLLLGT GKSPRKSVFF YPPYPDEIHG VFAVRNGKYK AHFFTQGSAH SDTTSDPACH AANRLTAHEP PLLYDLSQDP GENYNVLESI EGVSPEALQA LKHIQLLKAQ YDAAMTFGPS QIAKGEDPAL QICCQPSCTP HPVCCHCPGS QSHHHHHH