Recombinant mouse CD200R1 protein

Cd200r1, also known cell surface glycoprotein CD200 receptor 1, is an isoform of CD200 receptors which is expressed on cells of the myeloid lineage. Its inhibitory signaling pathway has been implicated in playing a prominent role in limiting inflammation in a wide range of inflammatory diseases. Its signaling inhibits the expression of proinflammatory molecules including tumor necrosis factor, interferons, and inducible nitric oxide synthase in response to selected stimuli. Recombinant mouse Cd200r1, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human CD200 (CAT# ATGP3964) is coated at 1 µg/mL (100 ul/well) can bind Human CD200 R1. The ED50 range ≤ 1 µg/mL.

Product Name Alternative

Cell surface glycoprotein CD200 receptor 1, Cd200r1, CD200R, Mox2r, OX2R, CD200 receptor 1, CD200 cell surface glycoprotein receptor, Cell surface glycoprotein OX2 receptor 1, CRTR2, MOX2 receptor

Expression System

Baculovirus

Antigen Species

Mouse

Tag

HIgG-His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Mouse CD200 (CAT# ATGP3964) . The ED50 range ≤ 1 µg/mL.

Molecular Weight

50.4 kDa (455aa)

Additionnal Information

CD200 R1, Cell surface glycoprotein CD200 receptor 1, Cd200r1, CD200R, Mox2r, OX2R, ATGP3457-10 µg, ATGP3457-20 µg, ATGP3457-50 µg, ATGP3457-100 µg, ATGP3457-250 µg, ATGP3457-500 µg, ATGP3457-1 mg, ATGP3457-10, ATGP3457-20, ATGP3457-50, ATGP3457-100, ATGP3457-250, ATGP3457-500, ATGP3457-1

References & Citations

Vaine CA., et al. (2014) Adv Immunol. 121:191-211.; ; Horie S., et al. (2013) Sci Rep. 3:3072.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_067300

Uniprot Accession Number

Q9ES57

Species

Mouse

N Terminal Sequence

Ala

AA Sequence

TDKNQTT QNNSSSPLTQ VNTTVSVQIG TKALLCCFSI PLTKAVLITW IIKLRGLPSC TIAYKVDTKT NETSCLGRNI TWASTPDHSP ELQISAVTLQ HEGTYTCETV TPEGNFEKNY DLQVLVPPEV TYFPEKNRSA VCEAMAGKPA AQISWSPDGD CVTTSESHSN GTVTVRSTCH WEQNNVSDVS CIVSHLTGNQ SLSIELSRGG NQSLRP