Recombinant human CNTF protein

CNTF (Ciliary neurotrophic factor) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. This protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. CNTF protein is structurally related to leukemia inhibitory factor (LIF), interleukin-6 (IL-6), interleukin-11 (IL-11) and oncostatin M (OSM) . It is localized in the cell nucleus subsequent to receptor binding. Recombinant human CNTF, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human CNTF in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 1 µg/mL.

Product Name Alternative

Ciliary neurotrophic factor

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 1 µg/mL.

Molecular Weight

25 kDa (220aa) confirmed by MALDI-TOF

Additionnal Information

CNTF, Ciliary neurotrophic factor, ATGP3455-10 µg, ATGP3455-20 µg, ATGP3455-50 µg, ATGP3455-100 µg, ATGP3455-250 µg, ATGP3455-500 µg, ATGP3455-1 mg, ATGP3455-10, ATGP3455-20, ATGP3455-50, ATGP3455-100, ATGP3455-250, ATGP3455-500, ATGP3455-1

References & Citations

Sendther M., et al. (1994), J Neurobiol. 25 (11) :1436-53.; ; Robert F., et al. (2007), J. Biol. Chem. 282 (46) : 33421-33434

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 1mM DTT, 30% glycerol, 0.2M NaCl

Scientific Category

Neuroscience

NCBI Accession Number

NP_000605

Uniprot Accession Number

P26441

Species

Human

AA Sequence

MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM