Recombinant human ADAM10 protein

ADAM10, as known as disintegrin and metalloproteinase domain-containing protein 10 isoform 1, is a member of the ADAM family that contains a disintegrin and metallogrotease-like domain. This protein is a sheddase, and has a broad specificity for peptide hydrolysis reactions. Also, it cleaves ephrin, within the ephrin/eph complex, formed between two cell surfaces. When ephrin is freed from the opposing cell, the entire ephrin/eph complex is endocytosed. In neurons, ADAM10 is the most important enzyme with alpha-secretase activity for proteolytic processing of the amyloid precursor protein. Recombinant human ADAM10, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Disintegrin and metalloproteinase domain-containing protein 10 isoform 1, ADAM10, AD10, AD18, CD156c, HsT18717, kuz, MADM, RAK

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 85% by SDS-PAGE

Molecular Weight

51.6 kDa (467aa)

Additionnal Information

ADAM10, Disintegrin and metalloproteinase domain-containing protein 10 isoform 1 , ADAM10, AD10, AD18, CD156c, HsT18717, kuz, MADM, RAK, ATGP3448-10 µg, ATGP3448-20 µg, ATGP3448-50 µg, ATGP3448-100 µg, ATGP3448-250 µg, ATGP3448-500 µg, ATGP3448-1 mg, ATGP3448-10, ATGP3448-20, ATGP3448-50, ATGP3448-100, ATGP3448-250, ATGP3448-500, ATGP3448-1

References & Citations

Park GB., et al, (2015) Invest. Ophthalmol. Vis. Sci. 56:5162-5173.; ; Zingoni A., et al, (2015) J. Immunol. 195:736-748.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Apoptosis

NCBI Accession Number

NP_001101

Uniprot Accession Number

O14672

Species

Human

AA Sequence

TTSAEKNTCQ LYIQTDHLFF KYYGTREAVI AQISSHVKAI DTIYQTTDFS GIRNISFMVK RIRINTTADE KDPTNPFRFP NIGVEKFLEL NSEQNHDDYC LAYVFTDRDF DDGVLGLAWV GAPSGSSGGI CEKSKLYSDG KKKSLNTGII TVQNYGSHVP PKVSHITFAH EVGHNFGSPH DSGTECTPGE SKNLGQKENG NYIMYARATS GDKLNNNKFS LCSIRNISQV LEKKRNNCFV ESGQPICGNG MVEQGEECDC GYSDQCKDEC CFDANQPEGR KCKLKPGKQC SPSQGPCCTA QCAFKSKSEK CRDDSDCARE GICNGFTALC PASDPKPNFT DCNRHTQVCI NGQCAGSICE KYGLEECTCA SSDGKDDKEL CHVCCMKKMD PSTCASTGSV QWSRHFSGRT ITLQPGSPCN DFRGYCDVFM RCRLVDADGP LARLKKAIFS PELYENIAEL EHHHHHH