Recombinant human ADAM10 protein
ADAM10, as known as disintegrin and metalloproteinase domain-containing protein 10 isoform 1, is a member of the ADAM family that contains a disintegrin and metallogrotease-like domain. This protein is a sheddase, and has a broad specificity for peptide hydrolysis reactions. Also, it cleaves ephrin, within the ephrin/eph complex, formed between two cell surfaces. When ephrin is freed from the opposing cell, the entire ephrin/eph complex is endocytosed. In neurons, ADAM10 is the most important enzyme with alpha-secretase activity for proteolytic processing of the amyloid precursor protein. Recombinant human ADAM10, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Disintegrin and metalloproteinase domain-containing protein 10 isoform 1, ADAM10, AD10, AD18, CD156c, HsT18717, kuz, MADM, RAK
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 85% by SDS-PAGE
Molecular Weight
51.6 kDa (467aa)
Additionnal Information
References & Citations
Park GB., et al, (2015) Invest. Ophthalmol. Vis. Sci. 56:5162-5173.; ; Zingoni A., et al, (2015) J. Immunol. 195:736-748.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Apoptosis
NCBI Accession Number
NP_001101
Uniprot Accession Number
O14672
Species
Human
AA Sequence
Frequently Asked Questions
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