Recombinant human CD34 protein

CD34, also known as hematopoietic progenitor cell antigen CD34 isoform b, is a member of a family of single-pass transmembrane sialomucin proteins. It is adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. This protein could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Recombinant human CD34, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Hematopoietic progenitor cell antigen CD34 isoform b, CD34

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by BCA assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

28.6 kDa (268aa)

Additionnal Information

CD34, Hematopoietic progenitor cell antigen CD34 isoform b, CD34, ATGP3447-10 µg, ATGP3447-20 µg, ATGP3447-50 µg, ATGP3447-100 µg, ATGP3447-250 µg, ATGP3447-500 µg, ATGP3447-1 mg, ATGP3447-10, ATGP3447-20, ATGP3447-50, ATGP3447-100, ATGP3447-250, ATGP3447-500, ATGP3447-1

References & Citations

Nielsen JS., et al. (2009) Microcirculation. 16:487-496.; ; Mastrandrea F., et al. (2008) Eur Ann Allergy Clin Immunol. 40:90-103.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_001764.1

Uniprot Accession Number

P28906

Species

Human

N Terminal Sequence

Ala

AA Sequence

SLDNNGT ATPELPTQGT FSNVSTNVSY QETTTPSTLG STSLHPVSQH GNEATTNITE TTVKFTSTSV ITSVYGNTNS SVQSQTSVIS TVFTTPANVS TPETTLKPSL SPGNVSDLST TSTSLATSPT KPYTSSSPIL SDIKAEIKCS GIREVKLTQG ICLEQNKTSS CAEFKKDRGE GLARVLCGEE QADADAGAQV CSLLLAQSEV RPQCLLLVLA NRTEISSKLQ LMKKHQSDLK KLGILDFTEQ DVASHQSYSQ KT