Recombinant human IL-7 protein

IL7, as known as interleukin-7 isoform 1, is a cytokine of the hemopoietin family that plays important roles in lymphocyte differentiation, proliferation, and survival. This protein is a hematopoietic growth factor secreted by stromal cells in the bone marrow and thymus. It is also produced by keratinocytes, dendritic cells, hepatocytes, neurons, and epithelial cells but is not produced by normal lymphocytes. Recombinant human IL7, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Interleukin-7 isoform 1, IL7, IL-7, Il7, Interleukin-7

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

18.4 kDa (161aa)

Additionnal Information

Interleukin-7 isoform 1, IL 7, IL7, IL-7, Interleukin-7, Interleukin7, Interleukin 7, ATGP3442-10 µg, ATGP3442-20 µg, ATGP3442-50 µg, ATGP3442-100 µg, ATGP3442-250 µg, ATGP3442-500 µg, ATGP3442-1 mg, ATGP3442-010, ATGP3442-020, ATGP3442-050, ATGP3442-100, ATGP3442-250, ATGP3442-500, ATGP3442-01M

References & Citations

Iolyeva M., et al. (2013) Blood 122:2271-2281.; ; Younas M., et al. (2013) J. Immunol. 191:3161-3168.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_000871.1

Uniprot Accession Number

P13232

Species

Human

N Terminal Sequence

Ala

AA Sequence

DCDIEGK DGKQYESVLM VSIDQLLDSM KEIGSNCLNN EFNFFKRHIC DANKEGMFLF RAARKLRQFL KMNSTGDFDL HLLKVSEGTT ILLNCTGQVK GRKPAALGEA QPTKSLEENK SLKEQKKLND LCFLKRLLQE IKTCWNKILM GTKEH