Recombinant human AGA protein

AGA, as known as N (4) - (beta-N-acetylglucosaminyl) -L-asparaginase isoform 1, belongs to the N-terminal nucleophile (Ntn) hydrolase superfamily. This protein consists of different S100 proteins and then plays various roles in regulation of protein phosphorylation, the dynamics of cytoskeleton constituents, transcription factors, enzyme activities, cell growth and differentiation, and inflammatory response. Recombinant human AGA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

N (4) - (beta-N-acetylglucosaminyl) -L-asparaginase isoform 1, AGA, AGU, ASRG, GA

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

35.7 kDa (332aa)

Additionnal Information

AGA, N (4) - (beta-N-acetylglucosaminyl) -L-asparaginase isoform 1, AGA, AGU, ASRG, GA, ATGP3440-10 µg, ATGP3440-20 µg, ATGP3440-50 µg, ATGP3440-100 µg, ATGP3440-250 µg, ATGP3440-500 µg, ATGP3440-1 mg, ATGP3440-10, ATGP3440-20, ATGP3440-50, ATGP3440-100, ATGP3440-250, ATGP3440-500, ATGP3440-1

References & Citations

Saarela J., et al. (2004) Biochem. J. 378:363-371.; ; Ikonen E., et al. (1992) Hum. Mutat. 1:361-365.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_000018

Uniprot Accession Number

P20933

Species

Human

N Terminal Sequence

Ala

AA Sequence

SSPLPLV VNTWPFKNAT EAAWRALASG GSALDAVESG CAMCEREQCD GSVGFGGSPD ELGETTLDAM IMDGTTMDVG AVGDLRRIKN AIGVARKVLE HTTHTLLVGE SATTFAQSMG FINEDLSTTA SQALHSDWLA RNCQPNYWRN VIPDPSKYCG PYKPPGILKQ DIPIHKETED DRGHDTIGMV VIHKTGHIAA GTSTNGIKFK IHGRVGDSPI PGAGAYADDT AGAAAATGNG DILMRFLPSY QAVEYMRRGE DPTIACQKVI SRIQKHFPEF FGAVICANVT GSYGAACNKL STFTQFSFMV YNSEKNQPTE EKVDCI