Recombinant human MMP-1 protein

MMP1, also known as interstitial collagenase isoform 1, contains 4 hemopexin-like domains and is a member of the matrix metalloproteinase (MMP) family. It is capable of degrading a wide range of extracellular molecules and a number of bioactive molecules. It plays a central role in cell proliferation, migration, differentiation, angiogenesis, apoptosis and host defences. Recombinant human MMP1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Interstitial collagenase isoform 1, MMP1, CLG, CLGN

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

53.1 kDa (460aa)

Additionnal Information

MMP1, Interstitial collagenase isoform 1, MMP1, CLG, CLGN, ATGP3439-10 µg, ATGP3439-20 µg, ATGP3439-50 µg, ATGP3439-100 µg, ATGP3439-250 µg, ATGP3439-500 µg, ATGP3439-1 mg, ATGP3439-10, ATGP3439-20, ATGP3439-50, ATGP3439-100, ATGP3439-250, ATGP3439-500, ATGP3439-1

References & Citations

Kumar JD., et al. (2015) Am J Physiol Gastrointest Liver Physiol. 309:G78-G86.; ; Yang H., et al. (2015) Biochemistry. 54:3631-3639.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM MES buffer (pH 5.5) containing 10 mM CaCl2, 100 mM NaCl, 0.05% Brij35, 30% glycerol

Scientific Category

Adhesion & Movement

NCBI Accession Number

NP_002412

Uniprot Accession Number

P03956

Species

Human

N Terminal Sequence

His18

AA Sequence

HSFPATLETQ EQDVDLVQKY LEKYYNLKND GRQVEKRRNS GPVVEKLKQM QEFFGLKVTG KPDAETLKVM KQPRCGVPDV AQFVLTEGNP RWEQTHLTYR IENYTPDLPR ADVDHAIEKA FQLWSNVTPL TFTKVSEGQA DIMISFVRGD HRDNSPFDGP GGNLAHAFQP GPGIGGDAHF DEDERWTNNF REYNLHRVAA HELGHSLGLS HSTDIGALMY PSYTFSGDVQ LAQDDIDGIQ AIYGRSQNPV QPIGPQTPKA CDSKLTFDAI TTIRGEVMFF KDRFYMRTNP FYPEVELNFI SVFWPQLPNG LEAAYEFADR DEVRFFKGNK YWAVQGQNVL HGYPKDIYSS FGFPRTVKHI DAALSEENTG KTYFFVANKY WRYDEYKRSM DPGYPKMIAH DFPGIGHKVD AVFMKDGFFY FFHGTRQYKF DPKTKRILTL QKANSWFNCR KNLEHHHHHH