Recombinant mouse Tyrosine Hydroxylase protein

Th, also known as tyrosine 3-monooxygenase, is a rate-limiting enzyme in catecholamine synthesis. It uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to DOPA. It regulates dopamine (DA) neurotransmission at the biosynthesis and reuptake steps. It plays an important role in the physiology of adrenergic neurons. It effects overexpression in lymphocytes on the differentiation and function of T helper cells. Recombinant mouse Th, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Tyrosine 3-monooxygenase, Th, tyrosine hydroxylase

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

57 kDa (507aa)

Additionnal Information

Th, Tyrosine 3-monooxygenase, Th, tyrosine hydroxylase, ATGP3438-10 µg, ATGP3438-20 µg, ATGP3438-50 µg, ATGP3438-100 µg, ATGP3438-250 µg, ATGP3438-500 µg, ATGP3438-1 mg, ATGP3438-10, ATGP3438-20, ATGP3438-50, ATGP3438-100, ATGP3438-250, ATGP3438-500, ATGP3438-1

References & Citations

Yamada K., et al. (1992) Histochemistry. 97:201-206.; ; Daubner SC., et al. (2011) Arch Biochem Biophys. 508:1-12.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Neuroscience

NCBI Accession Number

NP_033403.1

Uniprot Accession Number

P24529

Species

Mouse

N Terminal Sequence

Ala

AA Sequence

MPTPSAS SPQPKGFRRA VSEQDTKQAE AVTSPRFIGR RQSLIEDARK EREAAAAAAA AAVASAEPGN PLEAVVFEER DGNAVLNLLF SLRGTKPSSL SRALKVFETF EAKIHHLETR PAQRPLAGSP HLEYFVRFEV PSGDLAALLS SVRRVSDDVR SAREDKVPWF PRKVSELDKC HHLVTKFDPD LDLDHPGFSD QAYRQRRKLI AEIAFQYKQG EPIPHVEYTK EEIATWKEVY ATLKGLYATH ACREHLEAFQ LLERYCGYRE DSIPQLEDVS HFLKERTGFQ LRPVAGLLSA RDFLASLAFR VFQCTQYIRH ASSPMHSPEP DCCHELLGHV PMLADRTFAQ FSQDIGLASL GASDEEIEKL STVYWFTVEF GLCKQNGELK AYGAGLLSSY GELLHSLSEE PEVRAFDPDT AAVQPYQDQT YQPVYFVSES FSDAKDKLRN YASRIQRPFS VKFDPYTLAI DVLDSPHTIR RSLEGVQDEL HTLTQALSAI S